TABLE 5.
Transcriptional regulation of fadE-lacZ fusions by expression of plasmid-encoded FadR in fadR strains of E. coli and S. enterica
| Strain | Plasmid present | Plasmid-encoded FadR | β-Galactosidase activitya
|
|
|---|---|---|---|---|
| RB | RB-C18:1 | |||
| E. coli SI199 (MC1061 fadR fadE-lacZ) | None | None | 1121 ± 115 | 1109 ± 96 |
| pACYC177 | None | 1057 ± 94 | 1083 ± 87 | |
| pSH11 | E. coli | 58 ± 13 | 371 ± 52 | |
| pSH12 | S. enterica | 53 ± 11 | 141 ± 17 | |
| S. enterica SI226 (LT2 fadR fadE-lacZ) | None | None | 1095 ± 97 | 1066 ± 104 |
| pACYC177 | None | 1081 ± 105 | 1057 ± 89 | |
| pSH11 | E. coli | 64 ± 10 | 701 ± 68 | |
| pSH12 | S. enterica | 60 ± 12 | 382 ± 47 | |
a
β-Galactosidase activities are expressed as the amount of o-nitrophenol produced per min per mg of protein and are the averages of three separate experiments, each assayed in duplicate. RB-oleate is RB medium supplemented with 5 mM oleate (C18:1).