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. 2002 Nov;22(22):8067–8078. doi: 10.1128/MCB.22.22.8067-8078.2002

FIG. 5.

FIG. 5.

SNAP190 aa 1 to 90 can recruit TBP to the DNA when fused to a heterologous DNA binding domain. (A) The structures of the various fusion proteins are illustrated. HT, His Tag. (B) EMSA performed with probes containing three tandem copies of the G17 GAL4 DNA binding site (3 X G17) (3) inserted 5 bp upstream of the wild-type (lanes 1 to 14) or mutated (lanes 15 to 20) HIV-1 TATA box (see Materials and Methods for the sequences of the probes), and the proteins indicated above the lanes. The presence (+) or absence (−) of TBP and the amounts (indicated by the thickness of the triangle) of the proteins containing the GAL4 DNA binding domain (DBD) are shown over the gel. DBD, GAL4 DBD-HT; (1-90)DBD, (1-90)GAL4 DBD-HT (see panel A). Both the GAL4 DBD-HT and (1-90)GAL4 DBD-HT proteins were titrated over a threefold range. In lanes 17 and 18, the same amount of GAL4 DBD-HT was used as in lane 5. In lanes 19 and 20, the same amount of (1-90)GAL4 DBD-HT was used as in lane 11. The proteins present in the various protein-DNA complexes are indicated to the left of the panels.