FIG. 7.
TBP can be recruited to the U6 transcription initiation complex in the absence of SNAP190 aa 1 to 83. EMSA performed with the proteins indicated above the lanes and a probe containing the wild-type mouse U6 PSE and the human U6 TATA box. The amounts (indicated by the height of the triangle) of an anti-SNAP190 antibody (α-190) (CS696) and G-TBP, a GST-TBP fusion protein, are indicated over the lanes.The symbols to the right of different protein-DNA complexes indicate complexes containing proteins as follows: white circles, TBP and Brf2; blue circles, G-TBP and Brf2; white triangles, stmSNAPc#1 and TBP; blue triangles, stmSNAPc#1 and G-TBP; red triangle, stmSNAPc#1, TBP, and anti-SNAP190; white diamonds, stmSNAPc#1, TBP, and Brf2; blue diamonds, stmSNAPc#1, G-TBP, and Brf2; red diamonds, stmSNAPc#1, TBP, Brf2, and anti-SNAP190. (B) EMSA performed with probes containing the wild-type mouse U6 PSE and either a wild-type or mutant human U6 TATA box as indicated above the lanes, and the proteins indicated above the lanes. The Brf2 titrations were over a threefold range. The symbols at the right of different protein-DNA complexes indicate complexes containing proteins as follows: white circles, TBP and Brf2; white triangles, stmSNAPc#1 and TBP; white diamonds, stmSNAPc#1 or stmSNAPc#8, TBP, and Brf2.