FIG. 1.

Transcription of speB is regulated during infection. The figure presents relative transcript abundances of speB. Comparisons are shown of bacteria grown in broth culture to midexponential or early stationary phase or collected at 1, 2, and 3 days postinfection from mice infected subcutaneously. Relative transcript abundance was determined by real-time RT-PCR, and transcript levels are reported as the level of transcript relative to that detected in broth-grown bacteria at midexponential phase. Data represent the means and standard deviations of three independent experiments for samples analyzed in duplicate. Statistically significant (P < 0.001) differences from the exponential-phase samples are indicated by an asterisk. A significant (P < 0.001) difference between day 3 and day 1 expression is indicated by two asterisks.