TABLE 2.

Genes displaying increased transcript abundance under both pH 7.5 and 0.15 M NaCl conditions^a

Locus	Gene	Putative function	Fold change in expression under the indicated conditionsb
			pH 7.5	0.15 M NaCl
SPy0145	spy0145	Putative endoribonuclease	2.17	2.39
SPy0165	spn	NAD glycohydrolase precursorc	5.73 (4.7)	5.23 (7.4)
SPy0166	spy0166	Hypothetical proteinc	6.56	5.02
SPy0167	slo	Streptolysin O precursorc	4.37 (2.8)	5.19 (3.7)
SPy0739	sagB	Streptolysin S biogenesisd	3.59 (3.4)	3.61 (3.2)
SPy0740	sagC	Streptolysin S biogenesisd	3.42	3.39
SPy0741	sagD	Streptolysin S biogenesisd	2.36	2.66
SPy0742	sagE	Streptolysin S biogenesisd	2.57	2.80
SPy0743	sagF	Streptolysin S biogenesisd	2.09	2.71
SPy0744	sagG	Streptolysin S biogenesisd	2.45	2.79
SPy0745	sagH	Streptolysin S biogenesisd	2.46	2.76
SPy0746	sagI	Streptolysin S biogenesisd	2.29	2.26
SPy0747	spy0747	Predicted extracellular nuclease	2.15	2.40
SPy0843	spy0843	Putative surface antigen	2.81	2.03
SPy1151	ldh	Putative l-lactate dehydrogenase	3.13	2.35
SPy1378	nrdF.2	Ribonucleotide diphosphate reductase subunit	4.19	2.01
SPy1718	spy1718	Putative esterase	2.49	2.36
Spy1738	manL	Putative mannose PTS IIABe	4.96 (5.9)	4.66 (2.3)
SPy1739	manM	Putative mannose PTS IICe	4.45	4.93
SPy1740	manN	Putative mannose PTS IIDe	4.81	5.02
SPy1939	spy1939	Hypothetical protein	2.57	2.06
SPy2019	mga	M-protein trans-acting positive regulator	2.88	2.36

^aGenomic loci, gene names, and predicted functions are based on current annotation of the genome of M1 SF370 of S. pyogenes (20) in the National Center for Biotechnology Information database. PTS, phosphotransferase system.

^bSeveralfold change in expression in cultures grown to early stationary phase in pH 7.5 buffered medium or 0.15 M added NaCl medium versus cultures grown in unmodified control medium as determined by DNA microarray and real-time RT-PCR. Real-time RT-PCR data (shown in parentheses) were not collected for all genes that appeared to be organized in a monocistronic operon or for genes that were differentially regulated less than threefold as shown by microarray analysis.

^cPutative operon.

^dPutative operon.

^ePutative operon.