TABLE 3.

Genes displaying decreased transcript abundance under both pH 7.5 and 0.15 M NaCl conditions^a

Locus	Gene	Putative function	Fold change in expression under the indicated conditionsb
			pH 7.5	0.15 M NaCl
SPy0912	spy0912	Hypothetical protein	−4.27 (−6.1)	−3.27 (−3.9)
SPy1060	spy1060	Putative PTS IID (mannose specific)	−2.51	−2.57
SPy1158	spy1158	Putative sphingosine kinase	−2.81	−2.72
SPy1405	spy1405	Hypothetical protein	−2.12	−2.04
SPy1557	msrA	Putative peptide methionine sulfoxide reductase	−3.23	−2.38
SPy1559	ccdA	Putative cytochrome c biogenesis protein	−2.28	−2.51
SPy1628	fmt	Putative methionyl tRNA formyltransferase	−2.95	−2.08
SPy1724	susA	Transcription termination-antitermination factor	−2.40	−2.63
SPy1725	spy1725	Conserved hypothetical protein	−2.77	−2.79
SPy1746	fabZ	Putative beta-hydroxyacyl-ACP dehydratase	−3.10	−2.46
SPy1759	dnaJ	Heat shock (chaperone) protein	−2.63	−2.08
SPy1760	dnaK	Heat shock protein 70	−2.12	−2.45
SPy1761	grpE	Putative Hsp-70 cofactor	−2.93	−2.57
SPy1763	hrcA	Putative heat shock transcription repressor	−2.70	−2.65
SPy1815	scrA	Putative sucrose PTS IIc	−7.03	−2.15
SPy1816	scrB	Putative sucrose-6-phosphate hydrolasec	−5.81	−2.44
SPy1915	salA	Lantibiotic precursor	−5.94 (−9.4)	−4.49 (−5.1)
SPy2033	spy2033	Hypothetical protein	−2.74	−2.45
SPy2037	spy2037	Conserved hypothetical proteind	−73.07	−9.10
SPy2039	speB	Pyrogenic exotoxin Bd	−31.63 (−363.3)	−6.19 (−273.2)
SPy2040	spy2040	Hypothetical proteind	−38.70	−5.24
SPy2041	spy2041	Hypothetical proteind	−17.75	−12.86
SPy2050	spy2050	Putative PTS IICe	−4.19	−2.22
SPy2051	spy2051	Putative PTS IIBe	−4.56	−2.29
SPy2147	spy2174	Hypothetical protein	−2.35	−2.49
SPy2169	spy2169	Putative membrane proteinf	−5.94 (−4.3)	−4.17 (−5.9)
SPy2170	spy2170	Hypothetical proteinf	−6.08	−4.15
SPy2193	spy2193	Conserved hypothetical protein	−2.32	−2.36

^aGenomic loci, gene names, and predicted functions are based on current annotation of the genome of M1 SF370 of S. pyogenes (20) in the National Center for Biotechnology Information database. PTS, phosphotransferase system.

^bSeveralfold change in expression in cultures grown to early stationary phase in pH 7.5 buffered medium or 0.15 M added NaCl medium versus cultures grown in unmodified control medium as determined by DNA microarray or real-time RT-PCR. Real-time RT-PCR data (shown in parentheses) were not collected for all genes that appeared to be organized in a monocistronic operon or for genes that were differentially regulated less than threefold as determined by microarray analysis.

^cPutative operon.

^dPutative operon.

^ePutative operon.

^fPutative operon.