TABLE 4.

Expression in stationary bacteria in C media and tissue relative to exponential culture results^a

Locus	Gene (putative function)	Fold change in expression under the indicated conditionsb
		Tissue	Unmodified mediumc	pH 7.5d	0.15 M NaCle
SPy2039	speB (cysteine protease)	1,083.10	588.56*	1.10	1.99
SPy1915	salA (lantibiotic precursor)	15.83	5.13*	−1.86	1.56
SPy0739	sagB (SLS biogenesis)	2.22	2.00*	5.53*	5.80*
SPy1065	spn (NAD glycohydrolase)	−1.11	−1.41*	3.30	3.91
SPy1738	manL (mannose PTS II)	−11.97	−8.11*	−1.06	−2.54*
SPy1378	nrdF.2 (ribonucleotide diphosphate reductase)	−4.16	−21.06*	−1.79	−20.58*
Spy1228	spy1228 (lipoprotein)	−6.58	2.26	7.14	1.85
SPy1109	malP (l-malate permease)	2.10	42.47*	−3.73	25.22*
SPy1815	scrA (sucrose PTS II)	7.30	7.23*	1.29	6.22*
SPy0149	ntpK (sodium ATPase)	−58.21	−10.14*	−9.94*	−1.58

^aGenomic loci, gene names, and predicted functions are based on current annotation of the genome of M1 SF370 of S. pyogenes (20) in the National Center for Biotechnology Information database.

^bSeveralfold change in transcript abundance relative to unmodified medium exponential culture as determined by real-time RT-PCR. Broth cultures were harvested at early stationary phase, and tissue samples were harvested from the ulcer of infected hairless mice 3 days after inoculation of wild-type bacteria. An asterisk denotes genes for which the transcript profiles show the same trend as in the tissue sample (either both up- or both down-regulated).

^cR, 0.996. (The calculation used to determine correlation coefficient values presented in the table is described in Materials and Methods.)

^dR, 0.114.

^eR, 0.007.