TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Rhodobacter sphaeroides | ||
| 2.4.1 | Wild type | W. Sistrom |
| JZ3534 | ΔhemF::Ω(Spr-Str) | This study |
| PRRA2 | ΔprrA::Ω(Spr-Str) | 7 |
| Escherichia coli | ||
| DH5αphe | DH5αphe::Tn10dCmr | 8 |
| HB101 | F− Δ(gtp-proA)62 leuB6 supE44 ara-14 galK2 lacY1 | 1 |
| Plasmids | ||
| pBBR1MCS2 | Kmr | 12 |
| pBSIISK+ | Apr | Stratagene |
| pCF1010 | Promoterless lacZ vector, Mob+ Tcr Spr-Str | 14 |
| pJZ84 | hemF::lacZ transcription fusion in pCF1010 | This study |
| pKS7 | hemF in pUI1087 | This study |
| pKS9 | hemF in pBBR1MCS2 | This study |
| pKS11 | Ω(Spr-Str) inserted into (ΔBamHI)pKS9 | This study |
| pKS13 | ΔhemF::Ω(Spr-Str) in pSUP202 | This study |
| pRK2013 | ColEI replicon, Tra+ of RK2, Kmr | 5 |
| pSUP202 | pBR325 derivative, Mob+ Apr Cmr Tcr | 23 |
| pUI1087 | pBSIISK+ with modified polylinker | 30 |
| pUI1638 | Source of Ω(Spr-Str) cassette | 8 |