TABLE 3.

Efficiency of sporulation of B. anthracis 34F2 derivative strains expressing the Rap proteins in the pHT315 multicopy plasmid^a

Plasmid	Insert	Viable cells	Spore count	% Sporulation
pHT315	None	3.2 × 108	3.4 × 107	10.6
pHT315-BXA0205	BXA0205	2.4 × 107	2.8 × 104	1.2 × 10−3
pHT315-BXA0205 phr	BXA0205 phr	2.4 × 108	1.5 × 107	5.8
pHT315-3790	BA3790	1.3 × 108	4.9 × 106	3.7
pHT315-3790 phr	BA3790 phr	2.2 × 108	1.2 × 107	5.4
pHT315-1582	BA 1582	2.5 × 108	1.5 × 107	6
pHT315-3016	BA 3016	2.2 × 108	1.1 × 107	5
pHT315-4060	BA 4060	3.1 × 108	1.3 × 107	4.2
pHT315-3523	BCE3523	1.8 × 108	1.5 × 107	8
pHT315-3523 phr	BCE3523 phr	2.2 × 108	1.9 × 107	9

^aGrowth was carried out in Schaeffer's sporulation medium for 30 hours at 37°C. Data are representative of three independent experiments.