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. 2006 Jan;188(2):515–524. doi: 10.1128/JB.188.2.515-524.2006

FIG. 6.

FIG. 6.

Summary of the Ω4406 promoter region. (A) Effects of mutations upstream of the Ω4406 promoter. The indicated changes were made in the context of DNA spanning from bp −100 to 320 bp relative to the Ω4406 transcriptional start site. Upward and downward arrows indicate that developmental lacZ expression was increased or decreased, respectively, and numbers indicate the maximum β-galactosidase specific activity observed for the mutant promoter region, expressed as a percentage of wild-type promoter activity measured in the same experiment (Table 2). (B) Summary of deletion analysis and the effect of a multiple-base-pair mutation in the C-box-like sequence. Deletions show that DNA between bp −533 and −100 exerts a fourfold negative effect on Ω4406 promoter activity and suggest that DNA between bp 50 and 140 exerts a threefold positive effect. A multiple-base-pair change in the C-box-like sequence centered at bp −52 (rectangle) indicates it exerts a ninefold positive effect. Open arrows indicate the position of an imperfect inverted repeat sequence that appears to be involved in negative regulation.