TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | φ80 lacZΔM15 ΔlacU169 recA1 endA1 hsdR17 supE44 thi-1 gyrA relA1 | 11 |
| M. xanthus | ||
| DK1622 | Wild type | 19 |
| LS203 | csgA653 | 40 |
| fruA::TcΩ5 | fruA::Tcr | 34 |
| DK11209 | ΔdevS | A. Garza and D. Kaiser |
| MPV1727-8 | attB::pREG1727 | 46 |
| MKV4 | attB::pKV4 (pREG1727 with 1.0-kb XhoI-BamHI fragment from pPV4406-1.0)a | This study |
| MKV6 | attB::pKV6 (pREG1727 with 150-bp XhoI-BamHI fragment from pKV3) | This study |
| MKV11 | attB::pKV11 (pREG1727 with 0.6-kb XhoI-BamHI fragment from pKV9) | This study |
| MKV20 | attB::pKV20 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV12) | This study |
| MPV251 | attB::pPV0251 (pREG1727 with 351-bp XhoI-BamHI fragment from pPV251) | This study |
| MPV182 | attB::pPV0182 (pREG1727 with 282-bp XhoI-BamHI fragment from pPV182) | This study |
| MKV31 | attB::pKV31 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV28) | This study |
| MKV32 | attB::pKV32 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV29) | This study |
| MKV33 | attB::pKV33 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV30) | This study |
| MKV37 | attB::pKV37 (pREG1727 with 240-bp XhoI-BamHI fragment from pKV34) | This study |
| MKV39 | attB::pKV39 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV36) | This study |
| MKV55 | attB::pKV55 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV46) | This study |
| MKV56 | attB::pKV56 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV47) | This study |
| MKV57 | attB::pKV57 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV48) | This study |
| MKV58 | attB::pKV58 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV49) | This study |
| MKV59 | attB::pKV59 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV50) | This study |
| MKV60 | attB::pKV60 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV51) | This study |
| MKV61 | attB::pKV61 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV52) | This study |
| MKV63 | attB::pKV63 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV53) | This study |
| MKV65 | attB::pKV65 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV62) | This study |
| MKV67 | attB::pKV67 (pREG1727 with 420-bp XhoI-BamHI fragment from pKV64) | This study |
| MKV4ΔCP | csgA653 attB::pKV4 | This study |
| MKV11ΔCP | csgA653 attB::pKV11 | This study |
| MKV4ΔF | fruA::TcrattB::pKV4 | This study |
| MKV11ΔF | fruA::TcrattB::pKV11 | This study |
| MKV4ΔdevS | ΔdevS attB::pKV4 | This study |
| MKV11ΔdevS | ΔdevS attB::pKV11 | This study |
| Plasmids | ||
| pCR2.1-TOPO | Apr Kmr; lacZα | Invitrogen |
| pREG1727 | Apr Kmr; P1-inc attP lacZ | 7 |
| pPV4406-1.0 | pCR2.1-TOPO with 1.0-kb Ω4406 DNA segment from bp −533 to 485 generated by PCR | 32 |
| pKV3 | pCR2.1-TOPO with Ω4406 DNA segment from bp −100 to 50 generated by PCR | This study |
| pKV9 | pCR2.1-TOPO with 0.6-kb Ω4406 DNA segment from bp −100 to 485 generated by PCR | This study |
| pKV12 | pCR2.1-TOPO with Ω4406 DNA segment from bp −100 to 320 generated by PCR | This study |
| pPV251 | pCR2.1-TOPO with Ω4406 DNA segment from bp −100 to 251 generated by PCR | This study |
| pPV182 | pCR2.1-TOPO with Ω4406 DNA segment from bp −100 to 182 generated by PCR | This study |
| pKV28 | pKV12 with CATCGTG to ACGATGT mutation from bp −55 to −49 | This study |
| pKV29 | pKV12 with GGACA to TTCAC mutation from bp −69 to −65 | This study |
| pKV30 | pKV12 with GAACC to TCCAA mutation from bp −64 to −60 | This study |
| pKV34 | pCR2.1-TOPO with Ω4406 DNA segment from bp −100 to 140 generated by PCR | This study |
| pKV36 | pKV12 with GGCGTTTCA to TTATGGGAC mutation from bp −90 to −82 | This study |
| pKV46 | pKV12 with C to A mutation at bp −55 | This study |
| pKV47 | pKV12 with A to C mutation at bp −54 | This study |
| pKV48 | pKV12 with T to G mutation at bp −53 | This study |
| pKV49 | pKV12 with C to A mutation at bp −52 | This study |
| pKV50 | pKV12 with G to T mutation at bp −51 | This study |
| pKV51 | pKV12 with T to G mutation at bp −50 | This study |
| pKV52 | pKV12 with G to T mutation at bp −49 | This study |
| pKV53 | pKV12 with GTC to TGA mutation from −59 to −57 bp | This study |
| pKV62 | pKV12 with T to G mutation at bp −56 | This study |
| pKV64 | pKV12 with C to A mutation at bp −61 | This study |
a
Where possible, the plasmid description is given in parentheses after the strain description.