TABLE 2.
Summary of activities of mutant Ω4406 promoters
| Promoter assayed | Avg maximum β-galactosidase specific activity during developmenta ± SD | % Control activity measured in the same experimentb ± SD |
|---|---|---|
| Vector (no insert) | 16 ± 5 | |
| bp −533 to 485 | 90 ± 32 | 100 ± 36 |
| Deletions | ||
| bp −100 to 485 | 368 ± 24 | 476 ± 7 |
| bp −100 to 320 | 272 ± 35 | 346 ± 13 |
| bp −100 to 251 | 182 ± 12 | 205 ± 7 |
| bp −100 to 182 | 220 ± 50 | 244 ± 23 |
| bp −100 to 140 | 199 ± 30 | 247 ± 15 |
| bp −100 to 50 | 57 ± 6 | 55 ± 11 |
| Mutations in the C box-like sequence | ||
| CATCGTG −55 to −49 ACGATGT | 31 ± 10 | 11 ± 32 |
| C −55 A | 161 ± 3 | 59 ± 2 |
| A −54 C | 797 ± 199 | 293 ± 25 |
| T −53 G | 24 ± 9 | 9 ± 38 |
| C −52 A | 176 ± 34 | 65 ± 19 |
| G −51 T | 33 ± 4 | 12 ± 12 |
| T −50 G | 98 ± 22 | 36 ± 22 |
| G −49 T | 568 ± 93 | 209 ± 16 |
| Mutations farther upstream | ||
| T −56 G | 350 ± 6 | 129 ± 2 |
| GTC −59 to −57 TGA | 160 ± 25 | 59 ± 16 |
| GAACC −64 to −60 TCCAA | 67 ± 22 | 25 ± 32 |
| C −61 A | 95 ± 31 | 35 ± 33 |
| GGACA −69 to −65 TTCAC | 835 ± 175 | 307 ± 21 |
| GGCGTTTCA −90 to −82 TTATGGGAC | 421 ± 23 | 155 ± 5 |
The maximum β-galactosidase specific activity in nanomoles of o-nitrophenyl phosphate per minute per milligram of protein (average ± 1 standard deviation) is listed for three independently isolated M. xanthus transformants (one determination each), except in the case of the vector control (one transformant, seven determinations) and the deletion with Ω4406 DNA from bp −100 to 320 (three transformants, two determinations each). Samples were assayed at 0, 6, 12, 18, 24, 30, 36, and 48 h during development.
Controls were included in each experiment. For the deletions, the controls were Ω4406 DNA from bp −533 to 485 and the vector with no insert. The maximum for each deletion is expressed as a percentage of the maximum observed for bp −533 to 485 in the same experiment, after subtraction from both values of the maximum observed for vector alone in that experiment. Likewise, the maximum for each mutation is expressed as a percentage of the maximum observed for bp −100 to 320 in the same experiment, after subtraction from both values of the maximum observed for vector alone in that experiment. The average percentage ±1 standard deviation is listed.