TABLE 5.
Transduction of polar and nonpolar pstB insertion mutations into RmG804, RmG212, RmG490, and RmG830
| Recipient | Transduction with donor phagea
|
|
|---|---|---|
| φRmK385 (nonpolar pstB mutation, phoB+) | φRmK386 (polar pstB mutation, phoB) | |
| RmG212 (Rm1021 lac) | + | + |
| RmG804 (Rm1021 pit310::Tn5) | + | − |
| RmG490 (Rm1021 phoC490::ΩSp) | − | + |
| RmG830 (Rm1021 phoC490::ΩSp pit310::Tn5) | − | − |
a
+, transductants obtained (frequency, >1 × 10−6/donor); −, no transductants obtained (frequency, <1 × 10−8/recipient). Transductants were selected on LB medium containing gentamicin. RmK385 and RmK386 carry lacZ-aacC1 cassette insertions in pstB (Fig. 1A). In RmK385, transcription of phoB is driven from the aacC1 promoter.