Plasmids |
|
|
pGP704-sacB28 |
Used for sucrose-based selection, Apr
|
Gary Schoolnik |
pGPVC0928 |
pGP704-sacB28 containing the SOEing PCR product of rbmA
|
This study |
pACYC177 |
Low-copy-number cloning vector, Apr
|
New England Biolabs |
prbmA
|
pACYC177 harboring wild-type copy of rbmA
|
This study |
pBAD/Myc-His B |
Arabinose-inducible expression vector with C-terminal Myc epitope and six-His tags |
Invitrogen |
prbmA-myc
|
pBAD/Myc-His B containing the coding region of rbmA
|
This study |
pRS415 |
lacZ-based cloning vector for transcriptional fusion studies, Apr
|
50 |
prbmA::lacZ
|
pRS415 containing rbmA upstream regulatory fragment fused to lacZ
|
This study |
pCC11 |
pRS415 containing vpsA upstream regulatory fragment fused to lacZ
|
8 |
pCC12 |
pRS415 containing vpsL upstream regulatory fragment fused to lacZ
|
8 |
pGP704::Tn7-GFP |
Mini-Tn7 vector harboring a constitutively expressed gfp cassette |
Gary Schoolnik |
pUX-BF13 |
Helper plasmid containing transposase gene for mini-Tn7
|
Gary Schoolnik |
Strains |
|
|
V. cholerae 92A1552 |
|
|
FY_Vc_0001 |
O1 E1 Tor, Inaba, smooth, Rifr
|
60 |
FY_Vc_0002 |
O1 E1 Tor, Inaba, rugose, Rifr
|
60 |
FY_Vc_0004 |
Rugose ΔlacZ
|
8 |
FY_Vc_0005 |
RΔvpsT ΔlacZ
|
8 |
FY_Vc_0006 |
RΔvpsR ΔlacZ
|
8 |
FY_Vc_0007 |
RΔvpsR ΔvpsT ΔlacZ
|
8 |
FY_Vc_102 |
SΔrbmA
|
This study |
FY_Vc_105 |
RΔrbmA
|
This study |
FY_Vc_222 |
Rugose-gfp
|
This study |
FY_Vc_224 |
RΔrbmA-gfp
|
This study |
E. coli
|
|
|
DH5α |
F′ endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 (φ80dlacΔM15) |
Promega |
CC118 (λ pir) |
D(ara-leu) araD DlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λ pir
|
21 |
S17-1 (λ pir) |
Tpr SmrrecA thi pro rK− mK+RP4:2-Tc:MuKm Tn7 λ pir
|
15 |