TABLE 1.
Bacterial strains and plasmids used in the present study
| Strain or plasmid | Relevant description | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α(λpir) (= CVC23) | Supplies R6K π protein | M. Waldor |
| SM10(λpir) (= CVC201) | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu (λpir) pro endA hsdA hsdR supF | 7 |
| BW25113 | lacIqrrnB3ΔlacZ4787 hsdR514 ΔaraBAD567 ΔrhaBAD568 | 18 |
| BW27750 (= BR8701) | BW25113 ΔaraFGH φ(ΔaraEp kan PCP18-araE): araE promoter replaced with constitutive promoter CP18 | 18 |
| V. cholerae strains | ||
| CVC209 | N16961Strr | M. Waldor |
| CVC239 | N16961StrrPBADlacI-eyfp λcI-ecfp araE tetR Spcr | This study |
| CVC243 | Same as CVC239 except for 64× lacO in chrII | This study |
| CVC244 | Same as CVC239 except for 64× λOL1 in chrI | This study |
| CVC269 | Same as CVC243 except for 64× λOL1 in chrI | This study |
| CVC270 | Same as CVC239 except for 64× lacO in chrI | This study |
| CVC271 | Same as CVC239 except for 128× lacO in chrI | This study |
| CVC272 | Same as CVC239 except for 64× λOL1 in chrII | This study |
| CVC300 | N16961StrrPCP18araE kan | This study |
| CVC301 | N16961StrrPCP18araE | This study |
| CVC250 | N16961recA::kan | D. Pal |
| Plasmids | ||
| pCP20 | pSC101rep(Ts) Ampr; source of FLP recombinase | 4 |
| pDS132 | R6K γori, mobRP4 sacB Cmr; suicide vector for conjugal transfer and integration | 31 |
| pLAU85 | pBAD24 encoding LacI-ECFP and FtsZ-EYFP; Ampr | 20 |
| pPS9 | Coordinates 1512931-1516681 of chrI cloned in pDS132 | This study |
| pPS10 | Coordinates 462660-464883 of chrII cloned in pDS132 | This study |
| pPS11 | pDS132 + vca0527::64× lacO Kanr | This study |
| pPS17 | pZC496 + araE Spcr | This study |
| pPS18 | Coordinates 311001-313800 of chrII cloned in pDS132 | This study |
| pPS19 | pDS132 PBADlacI-eyfp λcI-ecfp PCP18araE tetR Spcr | This study |
| pPS20 | pDS132 PCP18araE Kanr | This study |
| pPS30 | pUC19 + 128× lacO Kanr | This study |
| pPS33 | pDS132 + vca0527::128× lacO Kanr | This study |
| pPS41 | pDS132 + vc1418::64× λOL1 Ampr | This study |
| pPS42 | pDS132 + vca0527::64× λOL1 Ampr | This study |
| pPS50 | pDS132 + vc1418::64× lacO Kanr | This study |
| pRFB110 | pUC19 + 64× lacO Kanr | 9 |
| pRFB122 | pUC19 + 64× λOL1 Cmr | 9 |
| pZC496 | pSC101 PBADlacI-eyfp λcI-ecfp tetR Spcr | Don Biek |
| pZE12-luc | Source of Ampr cassette | 26 |