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TABLE 2.

Primers used in this study

Primer name Location Primer sequence
PS25 chrI CTGCTCTAGACAACGAAGAAGCTCAGCTCGCTCTGCATGC
PS26 chrI ACTACGAGCTCGGACTCATCAGGTTCATCACCCC
PS27 chrI CGAGTACTTAATTCAAGGGCGTGATATCGC
PS31 chrI CCACTATACAATTTTGTGAGTTCTTATACG
PS28 chrII CTGCTCTAGAGACACGGAAAATATCGGAGATCATCCGCCC
PS29 chrII ACTACGAGCTCCCTACTGCATGAGCTGCGTCAAGGTGTGC
PS30 chrII GGCCGCTACTGCAGAGATGATCACCGCACGGTACCG
PS32 chrII CACTACCCAGAAAGATTGAAAGAGCGTTACC
PS33 BR8701 CGCCCAAGCTTGGCGACCAACAATACTCAACAACTACG
PS34 BR8701 TAGAAGGCCTCCCAGCTCATTCCTCCCAGCAAACC
PS35 chrII AAAACTGCAGGGCGGGCGTTAGCCATTTGGGAGAAC
PS36 chrII ACATGCATGCCCTAACGGCGAAACGGTTGTGCTCG
PS37 Kan CAGTCATAGCCGAATAGCCT
PS38 Kan CGGTGCCCTGAATGAACTGC
PS43 Amp GTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAG
PS44 Amp GGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACC
RAF76 lacI CGAGGGCTAGCAGGAAACAGCTATGAAACCAGTAACGTTATACGATG
RAF58 λcI TGCATTGATGCCATTAAATAAAGCGCTAACGCCTGACTGC
RAF70 araE TTCCGCCTCAATATGACG