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. 2006 Feb;188(3):882–893. doi: 10.1128/JB.188.3.882-893.2006

FIG. 3.

FIG. 3.

RT-PCR analysis of comB mRNA expression in H. pylori 26695 wt and ΔcomB mutant strains. Lanes 1 through 9, H. pylori 26695 wt; lanes 10 through 12, 26695Δhp0015. RT-PCR was performed with H. pylori 26695 wt cDNA (RT) (see Materials and Methods) using primer pairs AK05/CH81 (lane 3), AK05/CH77 (lane 6), and CH82/CH83 (lane 9) and with H. pylori 26695Δhp0015 cDNA using primer pair CH78/CH79 (lane 12). As positive controls, analogous PCRs with chromosomal DNA (C) of H. pylori 26695 wt (lanes 1, 4, and 7) and H. pylori 26695Δhp0015 (lane 10) were performed. No amplification products were detected in the negative controls (lanes 2, 5, 8, and 11), where RNA only, without cDNA synthesis (R), served as a template for PCR. Lane M, DNA size marker.