TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or characteristicsa | Reference or source |
|---|---|---|
| H. pylori strains | ||
| P1 | H. pylori wild type, naturally competent | 26 |
| P12 | H. pylori wild type, naturally competent | 25 |
| J99 | Wild-type, genome-sequenced strain | 2 |
| 26695 | Wild-type, genome-sequenced strain | 52 |
| P149 | Wild-type, Mongolian gerbil-adapted strain | 29 |
| P224 | P12; deletion of hp0015 (comB2), cat insertion | This study |
| P225 | P12; deletion of hp0016 (comB3), cat insertion | This study |
| P226 | P1; deletion of hp0015-hp0017, aphA-3 insertion | This study |
| P227 | P1; deletion of hp0037 (comB6), cat insertion | This study |
| P228 | P1; deletion of region spanning hp0037 (comB6)-hp0042 (comB10) | This study |
| P229 | P1Δhp0015-hp0017 complemented with pHel2 plasmid carrying comB2-comB4 (pAK20) | This study |
| P230 | P1Δhp0015-hp0017 complemented with pHel2 plasmid carrying comB3-comB4 (pAK21) | This study |
| P231 | P1Δhp0015-hp0017 complemented with pHel2 plasmid carrying comB4 (pAK22) | This study |
| P232 | P1Δhp0037-hp0042 complemented with pHel2 plasmid carrying comB6-comB10 (pAK24) | This study |
| P233 | P1Δhp0037-hp0042 complemented with pHel2 plasmid carrying comB7-comB10 (pDHO46) | This study |
| P234 | P149[hp1421::TnHK9] Tn insertion at codon 20 | 29 |
| E. coli strains | ||
| CC118 | Δ(ara-leu)7697 ΔlacX74 ΔphoA20 galE galK thi rpsE rpoB argE(Am) recA1 | 31 |
| β2155 | thrB1004 pro thi strA hsdS lacZΔM15 (F′ lacZΔM15 lacIqtraD36 proA+ proB+) ΔdapA::erm (Ermr) pir::RP4 [::kan (Kmr) from SM10] | 17 |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15] Tn10 (Tetr) | Stratagene |
| Plasmids | ||
| pRB67 | pMin2 plasmid carrying phoA without start codon | This study |
| pAK03 | pBA with region hp0014-hp0018, primers AK04-AK05 | This study |
| pAK04 | pBA with hp0015::cat and flanking regions | This study |
| pAK05 | pBA with hp0016::cat and flanking regions | This study |
| pAK07 | pBA with region hp0036-hp0038, AK02-AK03 | This study |
| pAK08 | pBA with hp0037::cat and flanking regions | This study |
| pAK09a | pRB67 with hp0037 fragment AK12-AK13 (34 aa) | This study |
| pAK09b | pRB67 with hp0037 fragment AK12-AK14 (62 aa) | This study |
| pAK09c | pRB67 with hp0037 fragment AK12-AK15 (115 aa) | This study |
| pAK09d | pRB67 with hp0037 fragment AK12-AK16 (198 aa) | This study |
| pAK09e | pRB67 with hp0037 fragment AK12-AK23 (236 aa) | This study |
| pAK09f | pRB67 with hp0037 fragment AK12-AK18 (296 aa) | This study |
| pAK19 | pBluescriptIISK comB2-comB4 deletion plasmid, aphA-3 | This study |
| pAK20 | pDH80, comB2-comB4 | This study |
| pAK21 | pDH80, comB3-comB4 | This study |
| pAK22 | pDH80, comB4 | This study |
| pAK23 | pMin1, comB6-comB10 deletion plasmid, aphA-3 | This study |
| pAK24 | pDH80, comB6-comB10 | This study |
| pDHO36 | pMin1, comB7-comB10 deletion plasmid, aphA-3 | 17 |
| pDHO46 | pDH80, comB7-comB10 | 25 |
a
aa, amino acids.