FIG. 5.

Localization of ohr and ohrR promoters and alignment of OhrR binding box. (A) Primer extension of RNA extracted from uninduced (UN) and CuOOH-induced cultures. The experiment was performed with a ³²P-labeled oligonucleotide primer as described in Materials and Methods. The C, T, A, and G lanes of a dideoxy sequencing ladder using the same primer as that used for the primer extension are shown. The ohr and ohrR transcription start sites are marked by arrowheads in the primer extension autoradiographs and as “+1” in the accompanying sequence. Putative −35 and −10 regions are shown in boldface italics. The translation initiation codons (ATG) are in boldface. The putative OhrR box is underlined. (B) Alignment of putative OhrR binding sites from X. campestris (32), B. subtilis (10), and A. tumefaciens. The numbers indicate the number of intervening nucleotides.