FIG. 7.
OhrR binds the ohr and ohrR promoters. The results of DNA mobility shift assays with 32P-labeled ohr (A) and ohrR (B) promoter fragments and purified OhrR. F, free probe; P, a reaction containing purified OhrR and labeled probe. UD and UP indicate reactions containing 2 μg of unrelated DNA (pBBR1MCS-4 plasmid) and 1 μg of unlabeled promoter, respectively. C, reactions in which CuOOH (1.0 mM) was added to the binding reaction. If not indicated, the amount of purified OhrR in the binding reaction was 0.3 μM. B, bound probe.