TABLE 1.
Bacterial strains, plasmids, and primers used in this study
| Strain plasmid, or primer | Description or sequence (5′ to 3′) | Source or reference |
|---|---|---|
| A. tumefaciens strains | ||
| NTL4 | pTiC58-cured derivative of strain C58 ΔtetC58 | 18 |
| bcp1 | bcp1::pKNOCK-Gm | This study |
| bcp2 | bcp2::pKNOCK-Gm | This study |
| prx1 | prx1::pKNOCK-Gm | This study |
| prx2 | prx2::pKNOCK-Gm | This study |
| prx3 | prx3::pKNOCK-Gm | This study |
| ohr | ohr::pKNOCK-Km | This study |
| ohrR | ohrR::pKNOCK-Gm | This study |
| ohr bcp1 | ohr::pKNOCK-Km, bcp1::pKNOCK-Gm | This study |
| ohr prx1 | ohr::pKNOCK-Km, prx1::pKNOCK-Gm | This study |
| Plasmids | ||
| pKNOCK | Broad-host-range suicide vector; RP4 oriT, R6K γ-ori | 1 |
| pBBR1MCS-4 | Broad-host-range cloning vector; rep, mob, lacZα, ApR | 15 |
| pBcp1 | pBBR1MCS-4 containing A. tumefaciens bcp1 | This study |
| pBcp2 | pBBR1MCS-4 containing A. tumefaciens bcp2 | This study |
| pPrx1 | pBBR1MCS-4 containing A. tumefaciens prx1 | This study |
| pPrx2 | pBBR1MCS-4 containing A. tumefaciens prx2 | This study |
| pPrx3 | pBBR1MCS-4 containing A. tumefaciens prx3 | This study |
| pOhr | pBBR1MCS-4 containing A. tumefaciens ohr | This study |
| pOhrR | pBBR1MCS-4 containing A. tumefaciens ohrR | This study |
| pPohr | pUFR027 containing ohr::lacZ | This study |
| pPohrR | pUFR027 containing ohrR::lacZ | This study |
| Primers | ||
| BT485 | TGGAGCAGGAAAATGGAC | |
| BT486 | GCCTATGGCCGGGAGAGA | |
| BT487 | GAAGGAGTAAATGCCATG | |
| BT488 | TAAGCCCGCTTTATCAGG | |
| BT532 | ATCGGGTAAGTGAGGACC | |
| BT533 | ATTCGCACCGCGACCGGC | |
| BT536 | CTGCGCGTAAAGGGCAA | |
| BT537 | GAGCGTGACGTCGAGAAC | |
| BT538 | CGGAACAGCTTTTCGCGG | |
| BT539 | TTCTTCGGCTTTCTCACG | |
| BT546 | TAGGCGTCAGCGCCACCA | |
| BT547 | TCGACCCGATCGGGGCTCA | |
| BT574 | GGAGAAAGCACACTATGA | |
| BT575 | TTCGTTAGCAGCTTAGCC | |
| BT907 | ATCGACTTCAGCGCGCTC | |
| BT908 | TTCGGCGATCTTGCCATC | |
| BT909 | GCGAAAGAAAGGTAGAAT | |
| BT910 | GGCTGGATGGCCCCTCAG | |
| BT911 | CACCTCAATCTTGCGCTT | |
| BT912 | CACCCGCTTCAAACATTG | |
| BT913 | TGCCAGGGATGGAAATGC | |
| BT914 | ATTGGCCAAGACACTCAT | |
| BT1046 | TAAGAATGGGCTTGTTAAA | |
| BT1047 | GTCTCCTCGTGCCTGTACT | |
| BT1173 | CGCTTAGAGCGCACACCAA | |
| BT1174 | TCTCGAAAATCGCGACGCC | |
| BT1236 | TAATTGTACGCTATAAGG | |
| BT1317 | AGAACAGGAGACAAGACATC | |
| BT1318 | AACCGAGATCAGGCCGCAGC | |
| BT1319 | CTATTGCCTTTCGGTCAACG | |
| BT1320 | ACTGCTCCACCAGACCGTCA |