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Pf20 mRNA and protein expression in Spag6+/+ and Spag6−/− mice. (A) (upper two panels) Northern blot analysis of testicular RNA for Spag6 and Pf20; (lower panel) Western blot analysis for Pf20 and Akap82 in testicular extracts. (B) Western blot analysis of epididymal sperm from wild-type and Spag6-deficient mice for Spag6, Pf20, and Akap82.
