FIG. 8.

Pf20-WD can interact with Spag6 in vivo. The open reading frame of TB-RBP and the cDNA encoding WD repeats of Pf20 were cloned in frame into the pBD-GAL4cam plasmid. The open reading frames of TB-RBP and Spag6 and the cDNA encoding the truncated TB-RBP₂₀₅ mutant were cloned in frame into the pAD-GAL4 plasmid. Pairs of BD and AD plasmids were cotransformed and streaked on SD plates lacking leucine, tryptophan, and histidine with growth indicating interaction. (A) BD-TB-RBP + AD-TB-RBP; (B) BD-TB-RBP + AD-TB-RBP₂₀₅; (C) BD-TB-RBP + AD-Spag6; (D) BD-Pf20-WD + AD-Spag6; (E) BD-Pf20-WD + AD-TB-RBP; (F) empty segment. These studies confirmed an interaction of Spag6 and the Pf20 WD repeats.