TABLE 2.
Effect of spoVS and flgM mutations on polar septation, engulfment, and spore formation
| Genotype | Spores/ml | % Sporangia (t2)a | % Sporangia with bulges (t3)b | % Sporangia fused (t4) |
|---|---|---|---|---|
| Wild type (PY79) | 4 × 108 | 55 (775)c | 0 (347) | 84 (248) |
| spoVS | 1 × 106 | 6 (1,342) | 0 (547) | 46 (623) |
| spoIIB | 2 × 108 | 45 (1,670)d | 37 (699) | 7 (345) |
| spoVG | 9 × 108 | 41 (391)d | 0 (301) | 40 (251) |
| spoIIB spoVG | 9 × 104 | 55 (1,211) | 35 (1,073) | 0 (401) |
| spoIIB spoVG spoVS | 2 × 106 | 1 (312) | 6 (1,270) | 21 (1,459) |
| spoIIB spoVG flgMΔ80 | 2 × 106 | 30 (573) | 5 (761) | 9 (474) |
| spoIIB spoVG spoVS lytABCe | 5 × 105 | NDf | 13 (615) | 35 (764) |
| spoIIB spoVG flgMΔ80 lytABCe | 3 × 105 | NDf | 14 (336) | 34 (126) |
a
Percent sporangia is determined by dividing the number of sporangia by the total number of total cells (in parentheses) in the fields scored.
b
Percent sporangia with bulges or fused is calculated by dividing the number of sporangia with the indicated phenotype by the total number of sporangia (in parentheses).
c
The number in parentheses indicates the total number of cells scored (vegetative cells and sporangia).
d
Data for percent sporangia at t2 are from Perez et al. (23) and are included for comparison only.
e
The additional deletion of lytD had no effect on suppression (data not shown).
f
ND, not determined.