Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Pretreatment with AMS in intact cells blocks periplasmic cysteine replacements from subsequent PEGylation. Cells were reacted with (+) and without (−) AMS for 45 min before being washed and probed with mPEG under denaturing conditions as described in the text. Aer-mPEG adducts (Aer-mp) showed a mobility shift. The enlarged lanes in the lower panel are higher exposures taken from different Western blots; these highlight replacements 184 and 187, which were reproducibly blocked by AMS.
