FIG. 3.

Zymogram analysis of E. faecalis ATCC 19433 autolysins under acidic conditions. Identical samples of the total proteins present in E. faecalis grown to the late exponential phase were subjected to the electrophoretic system involving two separating gels, one containing O-acetylated E. faecalis peptidoglycan (+OAc) and the other containing de-O-acetylated material (prepared by mild-base hydrolysis) (−OAc). The arrows and asterisks indicate the positions of autolysins with less and more activity with O-acetylated peptidoglycan, respectively.