TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| E. coli strains | ||
| BW25113 | lacIqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | Barry Wanner |
| MC1000 | araD139 Δ(ara-leu) Δ(codB-lacI) galK16 galE15 relA1 rpsL spoT1 | E. coli Genetic Stock Center |
| EC100D pir-116 | mcrA Δ(mrr hsdRMS mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) galU galK rpsL nupG pir-116 | Epicentre |
| NovaBlue Singles | endA1 hsdR17(rK12− mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′ [proA+B+lacIqZΔM15::Tn10] | Novagen |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac F′ [proA+B+lacIqZΔM15::Tn10] | Stratagene |
| TP1000 | araD Δ(argF-lac)U169 rpsL relA flbB ptsF devC rbsR mobAB::kan | Tracy Palmer |
| JOEY19 | MC1000 (λRS45 ytfE-lacZ) | This work |
| JOEY24 | MC1000 (λRS45 ytfE-lacZ) yjeB::EZ-Tn5TM <R6Kγori/KAN-2> | This work |
| JOEY28 | MC1000 (λRS45 ytfE-lacZ) yjeB::EZ-Tn5TM <R6Kγori/KAN-2> | This work |
| JOEY30 | MC1000 (λRS45 ytfE-lacZ) yjeB::EZ-Tn5TM <R6Kγori/KAN-2> | This work |
| JOEY59 | MC1000 (λRS45 ytfE-lacZ) yjeB::kan | This work |
| JOEY60 | MC1000 yjeB::kan | This work |
| JOEY61 | MC1000 (λRS45 ytfE-lacZ) ΔyjeB | This work |
| JOEY62 | MC1000 ΔyjeB | This work |
| JOEY72 | MC1000 (λRS45 hmpA-lacZ) | This work |
| JOEY73 | MC1000 (λRS45 ygbA-lacZ) | This work |
| JOEY75 | MC1000 (λRS45 tehA-lacZ) | This work |
| JOEY76 | MC1000 ΔyjeB (λRS45 hmpA-lacZ) | This work |
| JOEY77 | MC1000 ΔyjeB (λRS45 ygbA-lacZ) | This work |
| JOEY79 | MC1000 ΔyjeB (λRS45 tehA-lacZ) | This work |
| JOEY83 | MC1000 (λRS45 yjeB-lacZ) | This work |
| JOEY84 | MC1000 ΔyjeB (λRS45 yjeB-lacZ) | This work |
| Plasmids | ||
| pRS415 | lacZ fusion vector | Valley Stewart |
| pSTBlue-1 | Cloning vector | Novagen |
| pKD4 | Barry Wanner | |
| pCP20 | Barry Wanner | |
| pGIT1 | 205-bp ytfE promoter fragment in pSTBlue-1 | This work |
| pGIT2 | 354-bp hmpA promoter fragment in pSTBlue-1 | This work |
| pGIT3 | 292-bp ygbA promoter fragment in pSTBlue-1 | This work |
| pGIT5 | 232-bp tehA promoter fragment in pSTBlue-1 | This work |
| pGIT8 | 205-bp ytfE promoter fragment with ΔA deletion in pSTBlue-1 | This work |
| pGIT9 | yjeB and its 5′ noncoding region cloned in pSTBlue-1 | This work |