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. 2006 Feb;188(3):863–873. doi: 10.1128/JB.188.3.863-873.2006

FIG. 6.

FIG. 6.

Electrophoretic mobility shift assays of CMD-1 Mga mutants binding to Mga-regulated promoters. (A) Electrophoretic mobility shift assays of Mga-regulated promoter Pemm. C-terminal 6X-His fusion proteins (Mga-His) were purified from E. coli lysates. Identical amounts of the radiolabeled promoter probe Pemm were incubated for 15 min at 16°C with an increasing amount (0.5, 5, and 10 μg) of either the wild-type (lanes 2 to 4 and 12 to 13), Q11R (lanes 5 to 7), R13A (lanes 8 to 10), W12R (lanes 14 to 16), E14A (lanes 17 to 19), or no M6 Mga-His (lanes 1 and 11) prior to separation on a 5% polyacrylamide gel. (B) Electrophoretic mobility shift assays of the native Pmga promoter were performed as described above using 0.5 and 5 μg of the wild type (lanes 2 and 3), Q11R (lanes 4 and 5), W12R (lanes 6 and 7), R13A (lanes 8 and 9), E14A (lanes 10 and 11), or no Mga-His protein (lane 1).