TABLE 1.
PCR primers and their relevant targetsa
| Target and primer | Sequence | Reference |
|---|---|---|
| 23S rRNA | ||
| rRNA-23SL | GGAAGGTAAGCCAAAGAGAG | 23 |
| rRNA-23SR | TCCTAGTTGTCTGTGCAACC | 23 |
| Pemm | ||
| Pemm-L1 | GCATGGATCCCATCGCAAAGAGCTTA | 16 |
| Pemm-R1 | GCGGCTCGAGTAGTGTCTATTCGTGTTATT | 16 |
| Pmga | ||
| OYL-25 | TACCATAAAATACCTTTC | 16 |
| OYR-25 | GGTTGTACCATAACAGTC | 16 |
| mga6 | ||
| Mga6-150 | gcgtcaaagcttctaATCTCCTGATACTTGTACGG | This study |
| Mga-Pet1_Nde | ggggcatATGTATGTAAGTAAGTTGTTT | 1 |
| Mga-Pet2_Xho | aactcgagAGTTGTGGAGGGGG | 1 |
| OYR-29 | AAACCAACGCCTATTTGACGCATAC | This study |
| RMut1-R2 | cccgcctcgagAAAGAAGGGTATACAAGG | This study |
| RMut2-L | TCGACCTGCAGgcatgcaaa | This study |
| Mutagenic mga6 | ||
| M6 mga Q10R-a | GTTGTTTACAAGTCgACAGTGGAGAGAACTAAAATTAATCTCATACGTAACGG | This study |
| M6 mga Q10R-b | CCGTTACGTATGAGATTAATTTTAGTTCTCTCCACTGTcGACTTGTAAACAAC | This study |
| M6 mga Q11A-a | GTTGTTTACAAGTCAAgcGTGGAGAGAACTAAAATTAATCTCATACGTAACGG | This study |
| M6 mga Q11A-b | CCGTTACGTATGAGATTAATTTTAGTTCTCTCCACgcTTGACTTGTAAACAAC | This study |
| M6 mga W12A-a | GTTGTTTACAAGTCAACAGgcGAGAGAACTAAAATTAATCTCATACGTAACGG | This study |
| M6 mga W12A-b | CCGTTACGTATGAGATTAATTTTAGTTCTCTCgcCTGTTGACTTGTAAACAAC | This study |
| M6 mga W12R-a | GTTGTTTACAAGTCAACAGcGGAGAGAACTAAAATTAATCTCATACGTAACGG | This study |
| M6 mga W12R-b | CCGTTACGTATGAGATTAATTTTAGTTCTCTCCgCTGTTGACTTGTAAACAAC | This study |
| M6 mga R13A-a | GTTTACAAGTCAACAGTGGgcAGAACTAAAATTAATCTCATACGTAACGG | This study |
| M6 mga R13A-b | CCGTTACGTATGAGATTAATTTTAGTTCTgcCCACTGTTGACTTGTAAAC | This study |
| M6 mga E14A-a | GTTGTTTACAAGTCAACAGTGGAGAGcACTAAAATTAATCTCATACGTAACGG | This study |
| M6 mga E14A-b | CCGTTACGTATGAGATTAATTTTAGTgCTCTCCACTGTTGACTTGTAAACAAC | This study |
| M6 mga L15A-a | GTTTACAAGTCAACAGTGGAGAGAAgcAAAATTAATCTCATACGTAACGG | This study |
| M6 mga L15A-b | CCGTTACGTATGAGATTAATTTTgcTTCTCTCCACTGTTGACTTGTAAAC | This study |
| Mutagenic mga4 | ||
| M4 mga Q11R-a | GCATGTAAGTAAATTGTTTACTAGCCAACgATGGAGAGAATTGAAACTG | This study |
| M4 mga Q11R-b | CAGTTTCAATTCTCTCCATcGTTGGCTAGTAAACAATTTACTTACATGC | This study |
| M4 mga R13A-a | GTTTACTAGCCAACAATGGgcAGAATTGAAACTGATTTCATATTTAACAG | This study |
| M4 mga R13A-b | CTGTTAAATATGAAATCAGTTTCAATTCTgcCCATTGTTGGCTAGTAAAC | This study |
| M4 mga HTH4-a | GCTGAAGAGCTGTTTGTCAGCgcAgCTACCCTCAAACGCC | This study |
| M4 mga HTH4-b | GGCGTTTGAGGGTAGcTgcGCTGACAAACAGCTCTTCAGC | This study |
| Mutagenic dmgB | ||
| dmgB Q11R-a | CTCTTTACAACAAAACgGTGGAGAGAATTGGAGCTAATTGCGC | This study |
| dmgB Q11R-b | GCGCAATTAGCTCCAATTCTCTCCACcGTTTTGTTGTAAAGAG | This study |
| dmgB R13A-a | CTCTTTACAACAAAACAGTGGgcAGAATTGGAGCTAATTGCGC | This study |
| dmgB R13A-b | GCGCAATTAGCTCCAATTCTgcCCACTGTTTTGTTGTAAAGAG | This study |
| dmgB L42A-a | GTGTGAGAGATTAAACTGCTCACTCgcAACTTTACAATCATGTG | This study |
| dmgB L42A-b | CACATGATTGTAAAGTTgcGAGTGAGCAGTTTAATCTCTCACAC | This study |
| dmgB HTH4-a | GAGCTGTTTGTCAGCgcGgCAACACTCAAGCGTTTGATTG | This study |
| dmgB HTH4-b | CAATCAAACGCTTGAGTGTTGcCgcGCTGACAAACAGCTC | This study |
a
Mutagenic or noncomplementary sequences are in lowercase and introduced restriction sites are underlined.