Skip to main content
. 2002 Jan;184(1):142–151. doi: 10.1128/JB.184.1.142-151.2002

FIG. 8.

FIG. 8.

Separation of subcellular fractions of wild-type S. putrefaciens 200R and Fer− B31 via SDS-PAGE with heme (a) and Coomassie blue (b) staining. Lanes correspond to 200R outer membrane (lanes 1), 200R inner membrane (lanes 2), 200R soluble fraction (lanes 3), B31 outer membrane (lanes 4), B31 inner membrane (lanes 5), and B31 soluble fraction (lanes 6). The 91-kDa heme-containing band displaying Fe(III) reduction activity was excised from an unstained native gel and is included as a marker in lane I of both gels. Lanes II contain SDS-PAGE molecular mass standards (Bio-Rad) E. coli β-galactosidase (116,250 Da) and rabbit muscle phosphorylase b (97,400 Da).