FIG. 4.

Specificity profiles of native and recombinant plasminogen activators from S. uberis. Wells were cut in phosphate-buffered saline-agarose containing 1% skim milk. To these were added preincubated mixtures of plasminogen activator preparations such as WT PauA and WT PauB, wild-type plasminogen activators from culture supernatants of S. uberis 0140J and SK880, respectively; rPauA and rPauB, cleared whole-cell lysates from recombinant clones of PauA and PauB, respectively; SK(a), culture supernatant containing streptokinase from Group A streptococcus strain 0358) or a control recombinant lysate (rKan, encoding a kanamycin resistance gene) with mammalian plasminogen preparations designated as follows: H, human; R, rabbit; P, porcine; C, caprine; O, ovine; E, equine; B, bovine or a buffered saline control (pbs).