FIG. 2.

Primer extension analysis to determine the transcription start site of nfsA and ybjC. The primary extension product (large arrow) was generated from RNA isolated from paraquat-induced E. coli carrying pH3X1 by using the PRX primer. Secondary primer extension products are indicated by small arrows. The sequence shown to the left corresponds to the upper DNA strand depicted in Fig. 1 and is complementary to the sequence ladder (GATC) generated by dideoxy sequencing of pH3X1 by using the PRX primer. The transcription start site (+1) is in boldface and is indicated by a bent arrow. The −10 sequence is indicated.