TABLE 3.

Oligonucleotides used for PCR, sequencing, and mutagenesis

Name	Sequence (5′→3′)
T7 promoter	TAATACGACTCACTATAGGG
GerE-Seq1	GCTCTCACCTTAGCAAG
GerE-964F	GCCCTTCTTACATATGATATCTCG
GerE-5′For	GGAGATGAAAAAAGATGAAG
GerE-5′Rev	GCCAGTCTAGAGTAACCCTCCTTGCTAG
GerE-3′For	GCCGGGTACCGCTGTTTGTTTGGAACATTTCG
GerE-3′Rev	GCCGGAATTCGATGTATCCTGACAACACGTCTG
E5A-For	GAAGGAGAAAGCATTTCAATCG
E5A-Rev	CGATTGAAATGCTTTCTCCTTC
F6A-For	GAAGGAGAAAGAAGCTCAATCGAAGCCG
F6A-Rev	CGGCTTCGATTGAGCTTCTTTCTCCTTC
Q7A-For	GAGAAAGAATTTGCATCGAAGCCGTCG
Q7A-Rev	CGACGGCTTCGATGCAAATTCTTTCTC
S8A-For	GAAAGAATTTCAAGCGAAGCCGTCG
S8A-Rev	CGACGGCTTCGCTTGAAATTCTTTC
K9A-For	GAATTTCAATCGGCGCCGTCGCTAACG
K9A-Rev	CGTTAGCGACGGCGCCGATTGAAATTC
P10A-For	GAATTTCAATCGAAGGCGTCGCTAACG
P10A-Rev	CGTTAGCGACGCCTTCGATTGAAATTC
S11A-For	CAATCGAAGCCGGCGCTAACGAAAAG
S11A-Rev	CTTTTCGTTAGCGCCGGCTTCGATTG
K14A-For	GCCGTCGCTAACGGCAAGAGAAAGAGAAG
K14A-Rev	CTTCTCTTTCTCTTGCCGTTAGCGACGGC
Q25A-For	CGAATTGCTCGTTGCAGATAAGACAAC
Q25A-Rev	GTTGTCTTATCTGCAACGAGCAATTCG
K27A-For	GCTCGTTCAAGATGCGACAACAAAGGAG
K27A-Rev	CTCCTTTGTTGTCGCATCTTGAACGAGC
K30A-For	CAAGATAAGACAACAGCAGAGATTGCAAGCGAGC
K30A-Rev	GCTCGCTTGCAATCTCTGCTGTTGTCTTATCTTG
F37A-For	GATTGCAAGCGAGCTAGCTATCAGTGAGAAAACCG
F37A-Rev	CGGTTTTCTCACTGATAGCTAGCTCGCTTGCAATC
K41A-For	CTATTTATCAGTGAGGCAACCGTTCGAAAACC
K41A-Rev	GGTTTCGAACGGTTGCCTCACTGATAAATAG
R44A-For	GTGAGAAAACCGTTGCAAACCATATTTCAAATG
R44A-Rev	CATTTGAAATATGGTTTGCAACGGTTTTCTCAC
H46A-For	GAAAACCGTTCGAAACGCTATTTCAAATGCCATG
H46A-Rev	CATGGCATTTGAAATAGCGTTTCGAACGGTTTTC
Q52A-For	CAAATGCCATGGCGAAATTGGGTGTG
Q52A-Rev	CACACCCAATTTCGCCATGGCATTTG
L67A-For	GTTGTTGAGCTTGCTAGAATGGGTGAG
L67A-Rev	CTCACCCATTCTAGCAAGCTCAACAAC
E73A-For	GGGTGAGCTAGCGCTTTAATCCTTG
E73A-Rev	CAAGGATTAAAGCGCTAGCTCACCC
L74A-For	GGGTGAGCTAGAGGCTTAATCCTTGCCGG
L74A-Rev	CCGGCAAGGATTAAGCCTCTAGCTCACCC
AmyE-Back	ACCTGCATTTGCCAGCACAACGCC
CotC139-For	TGGGCCGATGAAAAATCGGCTC
CotC-Rev	TGTCGTAATCACAGTCATAATC
CotX-BcII	GAGCAGCTCATTGAACTGATCA
CotX-Hind	CGTGTTTTTCTTAAGCTTTCTC