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The kinetics of solubilization of PHE by AlnA. To quartz cuvettes containing 100 μg of crystalline PHE was added 1.0 ml of buffer containing 40 μg of AlnA, 40 μg of alasan, 40 μg of the 45-kDa protein, and 20 μg of E. coli OmpA. Absorbance was determined every 5 min without mixing.
