TABLE 1.

Enhanced solubilization of PHE^a

Addition	Solubility of PHE
	μg/mlb	Δμg/mlc	Δ μg/ml/mg of protein	Molecules of PHEd/molecule of protein
Buffer control	1.17
Alasan	3.54	2.37	59.3	9.9 (29.9)e
AlnA	4.42	3.25	81.2	16.3
45-kDa protein	4.15	2.98	74.5	18.8
E. coli OmpA	1.34	0.17	8.6	1.5

^aSolubility measurements were performed as described for Fig. 4.

^bCalculated from A₂₅₂ after equilibrium.

^cAfter subtracting solubility in the buffer control.

^dCalculated from molecular weights of PHE and the added protein. For alasan, it was assumed that the 16-, 31-, and 45-kDa proteins were present in equal molar quantities (31).

^eExpressed as molecules of PHE per molecule of the 45-kDa protein in the complex, assuming equal molar quantities of the 16-, 31-, and 45-kDa proteins.