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. 2002 Jan;184(1):82–90. doi: 10.1128/JB.184.1.82-90.2002

FIG. 2.

FIG. 2.

(A) Specific cystathionine lyase activity in CFE of L. lactis MG1363 cells grown in CDM with various methionine and cysteine concentrations. 0.1-0, 0.1× methionine, no cysteine; 1-0, 1× methionine, no cysteine; 10-0, 10× methionine, no cysteine; 0.1-1, 0.1× methionine, 1× cysteine; 10-1, 10× methionine, 1× cysteine. The specific activities are expressed as nanomoles of mercaptide formed per minute per milligram of protein. (B) Transcriptional analysis of the metC-cysK operon. Total RNA was extracted from L. lactis MG1363 cells grown in CDM with various methionine and cysteine concentrations (see panel A). The blot was hybridized with an internal gene fragment of MG1363 metC. The arrow indicates the position of the metC-cysK transcript. (C) Specific GUS activities in CFE of L. lactis MG1363 harboring pNZ9341 grown in CDM with various methionine and cysteine concentrations (see panel A). The specific activities are expressed as nanomoles of para-nitrophenyl-β-d-glucuronic acid converted per minute per milligram of protein.