TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Relevant characteristic(s)a | Reference |
|---|---|---|
| Strains | ||
| E. coli MC1061 | 6 | |
| L. lactis | ||
| MG1363 | Plasmid-free | 16 |
| MG1363ΔmetC | Eryr derivative of MG1363 with a metC disruption by single crossover | 12 |
| MG1363::pNZ9342 | Eryr derivative of MG1363 carrying a single chromosomal copy of the lacZ gene under the control of PmetC-cysK | This study |
| MG1363::ISS1#1 | Eryr Tetr derivative of MG1363::pNZ9342 with pGh8::ISS1 integrated in cmbR | This study |
| MG1363::ISS1#2 | Eryr Tetr derivative of MG1363::pNZ9342 with pGh8::ISS1 integrated in cmbR | This study |
| MG1363::ISS1#3 | Eryr Tetr derivative of MG1363::pNZ9342 with pGh8::ISS1 integrated in cmbR | This study |
| MG1363cmbR | Eryr derivative of MG1363 with a cmbR disruption by single crossover | This study |
| MG1363cmbT | Eryr derivative of MG1363 with a cmbT disruption by single crossover | This study |
| Plasmids | ||
| pGh8:ISS1 | Tetr, 5.2-kb pWV01 thermosensitive replicon | 28 |
| pNZ124 | Cmr, 2.8-kb pSH71 replicon | 37 |
| pUC19NcoI | Ampr, 2.6-kb pUC19 derivative containing an additional fragment with an NcoI site overlapping an ATG start codon preceded by the L. lactis SK11 prtP ribosome-binding site | This study |
| pNZ9340 | Cmr, 5.2-kb pNZ273 derivative containing the gusA gene fused to PmetC-cysK on a 0.6-kb ScaI-EcoRI fragment | This study |
| pNZ9341 | Eryr, 7.1-kb pIL252 derivative carrying the gusA gene fused to PmetC-cysK on a 0.6-kb ScaI-EcoRI fragment | This study |
| pNZ9342 | Eryr, 7.2-kb pUC19NcoI derivative containing the lacZ gene fused to PmetC-cysK and the Eryr gene from pIL253 | This study |
| pNZ9343 | Tetr, 5.5-kb pGh8:ISS1 derivative carrying a 0.3-kb EcoRI chromosomal DNA fragment | This study |
| pNZ9344 | Tetr, 5.5-kb pGh8:ISS1 derivative carrying a 0.3-kb HindIII chromosomal DNA fragment | This study |
| pNZ9345 | Tetr, 5.7-kb pGh8:ISS1 derivative carrying a 0.5-kb HindIII chromosomal DNA fragment | This study |
| pNZ9346 | Ampr, 6.3-kb pUC18 derivative carrying a 3.6-kb SalI-EcoRI fragment with cmbR | This study |
| pNZ9347 | Ampr, 9.5-kb pUC18 derivative carrying a 6.8-kb SstI-SalI fragment with cmbR | This study |
| pNZ9348 | Eryr, 4.0-kb pUC18Ery derivative carrying a 0.3-kb HindIII-AccI fragment of cmbR gene from pNZ9344 | This study |
| pNZ9349 | Eryr, 4.6-kb pUC18Ery derivative carrying a 0.8-kb PCR fragment of cmbT | This study |
| pNZ9350 | Cmr, 4.6-kb pNZ124 derivative carrying a 1.8-kb EcoRI-PstI fragment from with cmbR | This study |
a
Ampr, ampicillin resistant; Eryr, erythromycin resistant; Tetr, tetracycline resistant; Cmr, chloramphenicol resistant.