TABLE 1.
B. subtilis strains
| Strain | Genotype | Construction or referencea |
|---|---|---|
| Established strains | ||
| PY79 | spo+ | 38 |
| SC433 | SPβ::gerE-lacZ | 9 |
| SC1836 | spoIVBΔ::spc | 17 |
| NH578 | spoIVBΔ::spc amyE::spoIVB+ | pNH470 into SC1836 |
| NH577 | spoIVBΔ::spc amyE::pDG364 | pDG364 into SC1836 |
| Nonconservative mutants | ||
| NH1377 | spoIVBΔ::spc amyE::spoIVBDL213 | pNH1375 into SC1836 |
| NH1320 | spoIVBΔ::spc amyE::spoIVBHN236 | pNH1308 into SC1836 |
| NH1345 | spoIVBΔ::spc amyE::spoIVBDL240 | pNH1342 into SC1836 |
| NH1346 | spoIVBΔ::spc amyE::spoIVBDL242 | pNH1343 into SC1836 |
| NH1347 | spoIVBΔ::spc amyE::spoIVBNI290 | pNH1344 into SC1836 |
| NH1380 | spoIVBΔ::spc amyE::spoIVBKA321 | pNH1376 into SC1836 |
| NH1326 | spoIVBΔ::spc amyE::spoIVBDL363 | pNH1314 into SC1836 |
| NH1328 | spoIVBΔ::spc amyE::spoIVBSK378 | pNH1316 into SC1836 |
| NH1455 | spoIVBΔ::spc amyE::spoIVBKA387 | pNH1445 into SC1836 |
| NH1330 | spoIVBΔ::spc amyE::spoIVBHD394 | pNH1318 into SC1836 |
| Conservative mutants | ||
| NH1451 | spoIVBΔ::spc amyE::spoIVBHF236 | pNH1441 into SC1836 |
| NH1452 | spoIVBΔ::spc amyE::spoIVBDN242 | pNH1442 into SC1836 |
| NH1453 | spoIVBΔ::spc amyE::spoIVBDN363 | pNH1443 into SC1836 |
| NH1454 | spoIVBΔ::spc amyE::spoIVBSA378 | pNH1444 into SC1836 |
a
Mutations were created in pBluescript clones carrying the spoIVB gene, sequenced, and subcloned into pDG364 to give the plasmid clones indicated. These subclones were then linearized and DNA was introduced into the amyE locus of SC1836 cells as described in Materials and Methods.