TABLE 2.

spoIVB alleles

Straina	Relevant allele	Mutationb	Heat resistancec	Lysozyme resistancec	Germination phenotyped	Signalinge
NH577	spoIVBΔ::spc	Insertion-deletion	4.9 × 10−5	1.56 × 10−2	ND	−
NH578	spoIVB+	Wild type	88	54.4	+	+
NH1377	spoIVBDL213	Asp213 to Leu	98	94.5	+	+
NH1320	spoIVBHN236	His236 to Asn	1.18 × 10−4	1.12 × 10−2	ND	−
NH1451	spoIVBHF236	His236 to Phe	2.3 × 10−4	6.9 × 10−3	ND	−
NH1345	spoIVBDL240	Asp240 to Leu	95.7	55.7	+	+
NH1346	spoIVBDL242	Asp242 to Leu	1.12 × 10−3	2.19 × 10−2	ND	−
NH1452	spoIVBDN242	Asp242 to Asn	87.6	45	+	+
NH1347	spoIVBNI290	Asn290 to Ile	28.9	29	+ (30°C), − (37°C)	Delayed
NH1380	spoIVBKA321	Lys321 to Ala	86	88	+	+
NH1326	spoIVBDL363	Asp363 to Leu	1.54 × 10−4	3.7 × 10−2	ND	−
NH1453	spoIVBDN363	Asp363 to Asn	77.6	42	+	+
NH1328	spoIVBSK378	Ser378 to Lys	1.76 × 10−4	1.77 × 10−2	ND	−
NH1454	spoIVBSA378	Ser378 to Ala	3.25 × 10−4	3.26 × 10−3	ND	−
NH1455	spoIVBKA387	Lys387 to Ala	68.1	57	+	+
NH1330	spoIVBHD394	His394 to Asp	26.7	34.7	− (30°C), − (37°C)	Delayed

^aSee Table 1.

^bSee Fig. 1.

^cHeat or lysozyme resistance of cultures 24 h after the initiation of sporulation in DS medium (16). Values are expressed as percentages of CFU per milliliter in the untreated culture.

^dFive-day-old colonies grown on sporulation agar were tested for their capacity to germinate normally on tetrazolium agar plates as described previously (16). All strains that were phenotypically Spo⁻ were not tested for a germination phenotype (ND).

^eSignaling was defined according to the following two criteria: firstly, formation of pigmented colonies (Pig⁺) associated with the production of the ς^K-expressed CotA protein (30), and secondly, expression of the ς^K-controlled reporter gene, gerE-lacZ, in cells containing an SPβgerE-lacZ lysogen (see Fig. 2) as described previously (9).