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. 2002 Jan;184(1):29–42. doi: 10.1128/JB.184.1.29-42.2002

FIG. 2.

FIG. 2.

Complementation of S. cerevisiae vrg4 mutants by the CaVRG4 gene. (A) Complementation of hygromycin B-sensitive growth of the S. cerevisiae vrg4-2 mutant. Integrative plasmids expressing CaVRG4 (pTiM1) or ScVRG4 (pTαO-VRG4) (25) were introduced into NDY5 (vrg4-2), as described in Materials and Methods. Cells were plated on YPAD with or without 30 μg of hygromycin B per ml and incubated at 30°C for 3 days. (B) Complementation of the lethality associated with loss of VRG4 function, as assayed in the S. cerevisiae GAL1p-VRG4 strain. Integrative plasmids expressing CaVRG4 (pTiM1) or ScVRG4 (pTαO-VRG4) (25) were introduced into S. cerevisiae GAL1p-VRG4 strain XGY14 as described in Materials and Methods. Strains were incubated for 3 days on YPA plates containing galactose or glucose. (C) Integrative plasmids containing wild-type CaVRG4 (pTiM1) or the Cavrg4-A316D allele (pTiM1-A316D) introduced into S. cerevisiae vrg4-2 strain NDY5 and assayed for complementation of the hygromycin B-sensitive growth phenotype as described above.