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CaVRG4 is an essential gene. Heterozygous uracil prototrophic and auxotrophic strains ANC1 (VRG4/vrg4Δ::hisG-URA3-hisG) and ANC2 (VRG4/vrg4Δ::hisG) and the hemizygotic MET3p-VRG4 vrg4Δ::hisG strain ANC3 were plated on SD(−Ura) medium in the absence or in the presence of methionine and cysteine.
