FIG. 8.

Repression of VRG4 prevents hyphal formation on liquid and solid media. (A) C. albicans parental wild-type strain BWP17 and MET3p-VRG4 hemizygous strain ANC3 were precultured in SD(−Met/Cys), YPAD, or YPAD(+Met/Cys) for 12 h prior to induction of hyphal formation by growth at 37°C in YPAD containing 10% bovine calf serum. At zero time or 3 h after serum induction, aliquots were collected, stained with Calcofluor white, and examined by fluorescence microscopy. (B) Candida strains BWP17, ANC1 (VRG4/vrg4), and ANC3 were spotted on Spider medium plates and incubated for 3 days at 37°C. Individual cells from each colony were examined microscopically.