TABLE 1.
Plasmids used in this study
| Plasmid | Features | Reference or source |
|---|---|---|
| pAB-1 | lacZ gene under Ptac control in pMMB207 | 36 |
| pGS-flaA-01 | L. pneumophila flaA regulatory region in pUC18 | This study |
| pGS-flaA-02 | L. pneumophila flaA regulatory region in pMC1403 | This study |
| pGS-flaA-03 | L. pneumophila flaA regulatory region in pGS-lac-01 | This study |
| pGS-lac-01 | pAB-1 with a promoterless lacZ gene | This study |
| pGS-reg-F1 | Regulatory region of icmF in pUC18 | This study |
| pGS-reg-F2 | Regulatory region of icmF in pMC1403 | This study |
| pGS-reg-F3 | Regulatory region of icmF in pGS-lac-01 | This study |
| pGS-reg-M1 | Regulatory region of icmM in pUC18 | This study |
| pGS-reg-M2 | Regulatory region of icmM in pMC1403 | This study |
| pGS-reg-M3 | Regulatory region of icmM in pGS-lac-01 | This study |
| pGS-relA-01 | L. pneumophila relA gene in pUC-18 | This study |
| pGS-relA-01-Km | pGS-RelA-01 with the Km cassette in the relA gene | This study |
| pGS-relA-01-Km-GR | Insert of pGS-RelA-01-Km in pLAW344 | This study |
| pGS-relA-05 | L. pneumophila relA in pMMB207αb | This study |
| pKP-Q-1 | Regulatory region of icmQ in pUC18 | This study |
| pKP-Q-21 | Regulatory region of icmQ in pMC1403 | This study |
| pLAW344 | sacB MCS oriT(RK2) CmroriR(ColE1) Apr | 50 |
| pMC1403 | Promoterless lacZ in pBR322 | 8 |
| pMMB207αb | RSF1010 derivative, IncQ lacIq CmroriT MCS | 38 |
| pOG-J-103 | Regulatory region of icmJ in pUC18 | This study |
| pOG-J-109 | Regulatory region of icmJ in pMC1403 | This study |
| pOG-J-122 | Regulatory region of icmJ in pGS-lac-01 | This study |
| pOG-P-105 | Regulatory region of icmP in pUC18 | This study |
| pOG-P-108 | Regulatory region of icmP in pMC1403 | This study |
| pOG-P-121 | Regulatory region of icmP in pGS-lac-01 | This study |
| pOG-Q-126 | Regulatory region of icmQ in pGS-lac-01 | This study |
| pOG-R-125 | Regulatory region of icmR in pGS-lac-01 | This study |
| pOG-T-104 | Regulatory region of icmT in pUC18 | This study |
| pOG-T-107 | Regulatory region of icmT in pMC1403 | This study |
| pOG-T-120 | Regulatory region of icmT in pGS-lac-01 | This study |
| pOG-V-110 | Regulatory region of icmV in pMC1403 | This study |
| pOG-V-123 | Regulatory region of icmV in pGS-lac-01 | This study |
| pOG-VW-106 | Regulatory region of icmVW in pUC18 | This study |
| pOG-W-111 | Regulatory region of icmW in pMC1403 | This study |
| pOG-W-124 | Regulatory region of icmW in pGS-lac-01 | This study |
| pSS-R-1 | Regulatory region of icmR in pUC18 | This study |
| pSS-R-27 | Regulatory region of icmR in pMC1403 | This study |
| pUC18 | oriR(ColE1) MCS Apr | 53 |