FIG. 5.

Effects of glycogen synthesis and catabolism on biofilm formation at 24 h. Isogenic derivatives of strain MG1655, defective for glgA, csrA, or both, were compared. Plasmids pOP245, pJF02, or pOP12 carry glgA, glgP, or asd-glgBXCAP′ (deleted for most of glgP), respectively. (A) Effects of a polar glgA mutation. The strain identities for bars 1 to 7 were MG1655, glgA mutant, glgA mutant containing pOP245, pJF02, or both plasmids, the csrA mutant, and the csrA glgA double mutant, respectively. (B) Overexpression of glycogen biosynthetic or catabolic genes. Lanes 1 to 5 show MG1655 containing either no plasmid, pOP12, pBR322 (vector control), pJF02, or pUC19 (vector control), respectively. Each bar shows the averages and standard errors of three separate experiments. The asterisks denote significant differences with respect to the parent strain (P < 0.0001).