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. 2002 Jan;184(1):327–330. doi: 10.1128/JB.184.1.327-330.2002

FIG. 3.

FIG. 3.

Localization of mutant and wild-type VirB2 proteins in A. tumefaciens NT1RE. Strain NT1RE contained either pJK270 (wild-type virB2) (lanes 1 and 2), pUCD4605 (A45D-virB2) (lanes 3 and 4), pUCD4606 (ΔAAA-virB2) (lanes 5 and 6), or pUCD4607 (frameshift mutation virB2) (lanes 7 and 8). Isolated membrane vesicles from each strain were treated with 2% Sarkosyl to separate inner membrane (lanes 1, 3, 5, and 7)- and outer membrane (lanes 2, 4, 6, and 8)-associated proteins. Proteins were fractionated by Tricine-SDS-16.5% PAGE and analyzed by immunoblotting with anti-VirB2 antibody. Unprocessed and processed VirB2 proteins are indicated.