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Tumorigenicity assay on D. stramonium. Representative sections of the stem of each test plant inoculated with the indicated strain are shown at 4 to 5 weeks postinoculation. The inoculated strains of A. tumefaciens NT1RE contained either pJK270 (wild-type virB2 [wt-virB2]), pUCD4605 (A45D-virB2), pUCD4606 (ΔAAA-virB2), or pUCD4607 (virB2 frameshift mutant [fs-virB2]).
