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Point mutations in mutated Tnps as indicated by DNA sequencing for Tnp sA5 (best first-round papillator), sB2 (best second-round papillator), sC6 (best third-round papillator), sD5 (best fourth-round papillator), and sC7 (A). The transposition activity of four mutant Tnps was quantified by papillation assay (B). Tnp WT was also tested but failed to promote a single detectable transposition event.
