FIG. 4.

Normalized in vivo transposition activity of mutant Tnps with IE- and OE-defined transposon. The ability of each mutant Tnp to promote transposition of transposons with IE and OE sequences was quantified by the mating-out assay. While Tnp WT results in equal levels of transposition for each transposon, some mutants preferentially function with one end. Tnp sC7 is very hyperactive with IE-flanked transposons while functioning at a “normal” level when tested with OE-defined transposons. Transposition rates shown were obtained by dividing the transposition frequency for the mutant Tnp by the rate of Tnp WT activity (6.5 × 10⁻⁵).