In vitro transposition of OE- and IEME-defined transposons. Tnp sC7v2.0 and Tnp EK/LP activity with plasmid DNA containing transposons with either two OE or two IEME ends. Lane 1, 1-kb DNA ladder (Promega); lane 2, Tnp sC7v2.0 with OE-defined transposons; lane 3, Tnp EK/LP with OE-defined transposon; lane 4, Tnp sC7v2.0 with IEME transposons; lane 5, Tnp EK/LP with IEME transposons.