TABLE 3.

Mas activity and estimate of glyoxylate accumulation during growth on different carbon sources

Carbon source	Mas activity (U/mg of protein)a in strain:			Formation of phenylhydrazones from aldo- and ketoacidsb (OD324/OD600/ml of supernatant) in strain:
	D188-5	D188	X798	D188-5	D188	X798
Acetate	198.9 ± 50.9	234.4 ± 133.1	NDc	0.2*	0.3*	12.4†
Glycerol	52.1 ± 43.8	248.2 ± 71.7	ND	0.3*	0.2*	1.2‡
Plant	85.9 ± 26.9	103.5 ± 26.7	ND	0.4*	0.4*	1.1‡
Gall	137.3 ± 45.0	118.4 ± 21.0	ND	0.6*	0.4*	4.5§

^aThe data are averages of three independent experiments.

^bFor strains D188-5 and D188, the aldo- and ketoacid levels under the four growth conditions are not significantly different (P > 0.05; *); for mutant strain X798, there is a significant difference between aldo- and ketoacid levels on acetate (†), glycerol and plant (‡), and leafy gall (§) (P < 0.001). Also, between strains D188 and D188-5 and strain X798, there is a significant difference in aldo- and ketoacid concentrations (P < 0.001).

^cND, not detected.